Job Description

We are seeking a **_Research Specialist II_** to join the lab at Johns Hopkins All Children's Hospital in St. Petersburg, Florida. This position will work in a lab focused on cancer immunology research investigating the tumor microenvironment and anti-tumor immune responses, including organoid and primary cell culture systems and in vivo preclinical tumor models. The specialist will contribute to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment. The Research Specialist II develops assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel. **Specific Duties & Responsibilities** + Conduct assigned laboratory projects. + Utilize specialized knowledge to interpret results, e.g. read controls, evaluate results, etc. + Work independently and use scientific knowledge and lab experience to make judgments and decisions. + Teach/train learners, and new technicians in the laboratory. + Guide, direct, and facilitate problem solving for entry-level laboratory members on day-to-day problems with more challenging research assays. + Make scientific contributions to protocol development, efficiency, and productivity. + Collaborate in the development of new techniques and instruments. + Apply published research methods to current experimental work by investigating published protocols and discussing them with PI. + Scientifically contribute to the preparation of manuscripts and proposals. + Process analyzed data into figures for scientific publications. + Order equipment and supplies, interface with vendors. + Maintain daily records of experiments. + Organize and track laboratory samples. + Other duties as assigned. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field + Two years of laboratory experience performing relevant techniques including experimental techniques used in genomics, sequencing, and cancer biology assays. + Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Master's Degree with related graduate research. + Cell culture. + RNA isolation and quantitative PCR (qPCR). + Common cell biology assays. + Genomics and sequencing. + Immunostaining. + Western blotting. + FACS analysis. + Mouse handling. + Bioinformatics analysis. + GraphPad/Prism/Adobe. **Technical Skills & Expected Level of Proficiency** + Assay Development and Validation - Developing + Data Management and Analysis - Developing + Experimental Design and Execution - Developing + Operations Management - Developing + Protocol Development and Optimization - Developing + Scientific Writing - Developing + Stakeholder Engagement - Developing _The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs_ Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $41,300 - $72,300 Annually (Commensurate w/exp.) Employee group: Full Time Schedule: Mon-Fri, 8.30am-5.00pm FLSA Status: Exempt Location: Florida Department name: All Childrens Personnel area: School of Medicine Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

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